Multi-Target Amyloid Probing and Inhibition Using Basic Orange Fluorescence
نویسندگان
چکیده
Misfolding and aggregation of amyloid peptides are critical pathological events in numerous protein misfolding diseases (PMDs), such as Alzheimer’s disease (AD), type II diabetes (T2D), medullary thyroid carcinoma (MTC)....
منابع مشابه
Supravital Blood Studies, Using Acridine Orange Fluorescence
LTHOUGH FLUORESCENCE microscopy had its beginnings over half a century ago, it has only recently gained widespread usage as a research tool, and practical applications have yet to be fully exploited. Blood has been one of the tissues most extensively studied by fluorochroming,’ but few reports have been published in English. In addition, the previously reported saline dilution technics for supr...
متن کاملSupravital blood studies, using acridine orange fluorescence.
LTHOUGH FLUORESCENCE microscopy had its beginnings over half a century ago, it has only recently gained widespread usage as a research tool, and practical applications have yet to be fully exploited. Blood has been one of the tissues most extensively studied by fluorochroming,’ but few reports have been published in English. In addition, the previously reported saline dilution technics for supr...
متن کاملProbing Amyloid Aggregation and Morphology In Situ by Multiparameter Imaging and Super-Resolution Fluorescence Microscopy
Chapter Outline Introduction 105 Multi-Parameter Microscopy of Protein Aggregation Kinetics 106 Fusion Protein Labeling of Amyloidogenic Proteins 106 Principle of Multi-Parameter Imaging Techniques 107 Fluorescence Anisotropy Imaging 107 Principle of HomoFRET in the Study of Protein SelfAssembly Reactions 107 HomoFRET Imaging for the Study of α-synuclein Aggregation 108 Fluorescence Lifetime Im...
متن کاملAnalysis of glycosaminoglycans in urine by using acridine orange fluorescence.
The fluorescence technique described here utilizes the electrostatic interaction between the polyanionic sites of glycosaminoglycans and the cationic dye Acridine Orange to analyse urinary glycosaminoglycans from patients suffering from mucopolysaccharidoses. The basis of the titration is the decrease in the fluorescence of free Acridine Orange that occurs when it is bound to polyanions. The ef...
متن کاملInhibition of familial cerebral amyloid angiopathy mutant amyloid beta-protein fibril assembly by myelin basic protein.
Deposition of fibrillar amyloid beta-protein (Abeta) in the brain is a prominent pathological feature of Alzheimer disease and related disorders, including familial forms of cerebral amyloid angiopathy (CAA). Mutant forms of Abeta, including Dutch- and Iowa-type Abeta, which are responsible for familial CAA, deposit primarily as fibrillar amyloid along the cerebral vasculature and are either ab...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Sensors & diagnostics
سال: 2023
ISSN: ['2635-0998']
DOI: https://doi.org/10.1039/d3sd00124e